Enzyme mRNA

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Introduction Product List Product Features Application Q&A

Introduction

Enzymes are proteins produced by living cells that act as efficient catalysts for specific substrates and are the primary catalysts for material and energy changes involved in the metabolic processes of the body. Besides, the growth and development of organisms, reproduction, genetic movement, neurotransmission and other life activities are all closely related to the catalytic process of enzymes. Creative Biolabs is committed to providing you with high quality enzyme mRNAs that can be applied in various enzyme-related regulatory mechanism studies as well as enzyme-related gene therapy probes. Our pre-produced enzyme mRNAs are all stable and time-tested, designed by a team of dedicated experts. You can choose appropriate nucleoside modification according to project requirements, with 5' end caps and 3' poly(A) being available for the enzyme mRNA. Furthermore, enzyme mRNA products are synthesized under strict QC procedures on advanced IVTScrip™ platform that can absolutely ensure the purity and quality of the product you order.

Considering diversity of enzyme types and functions, Creative Biolabs also offers customized services for specific enzyme mRNAs. If you provide corresponding DNA template directly, we will provide IVT mRNA synthesis and optimisation services. You can also choose one-stop enzyme mRNA customization service, including template design, promoter selection, codon optimization, 5'-UTR and 3'-UTR optimization, 3' poly(A) length and 5' capping determination. Creative Biolabs is committed to providing pre-made and custom enzyme mRNA products at competitive prices, and to help you get ready for most downstream applications. If you are interested in our products, please feel free to contact us.

Product List

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Product Features

Strict Quality Control Standards
- Multiple quality control standards.
- High stability and batch-to-batch consistency.
- Excellent purity and stability profile.
Efficient and Time-saving
- Pre-designed capping and poly (A) tail modification.
- Shorter turnaround time.

Application

Genetic Disorders: For genetic disorders caused by the deficiency or malfunction of a specific enzyme, enzyme mRNA therapy offers a promising solution. By introducing mRNA sequences that encode the functional enzyme into the patient's cells, this approach can restore the missing or defective enzymatic activity. This has been explored in conditions like Phenylketonuria (PKU) and Lysosomal Storage Disorders (LSDs), where enzyme replacement can significantly ameliorate disease symptoms and improve quality of life. The mRNA-based therapy ensures that the enzyme is produced endogenously, potentially reducing the immune responses associated with direct protein infusion therapies.
Cancer: In the realm of oncology, enzyme mRNA can be utilized to stimulate the body's immune response against cancer cells. For instance, mRNA encoding enzymes involved in antigen processing and presentation can enhance the effectiveness of cancer vaccines. By increasing the presentation of cancer antigens on tumor cells, these vaccines can more effectively target and eliminate malignant cells.
Enzyme Replacement Therapy: Enzyme replacement therapy (ERT) is an effective treatment for many genetic disorders caused by enzyme deficiencies. Currently, most ERTs involve regular injections of the missing enzyme, but the development of safe and effective mRNA delivery methods could offer an alternative.
Protein Engineering: Enzyme mRNA is also instrumental in protein engineering and directed evolution experiments. By introducing mRNA sequences that encode variants of a particular enzyme, scientists can explore the structure-function relationship of these proteins. This enables the identification and optimization of enzymes with desired properties, such as increased catalytic efficiency, altered substrate specificity, or enhanced stability. Such engineered enzymes have vast applications in biotechnology, pharmaceuticals, and industrial processes.

Q&A

Q: What methods are used to quantify enzyme mRNA expression levels?

A: Quantitative real-time PCR (qRT-PCR) is the most commonly used method due to its sensitivity, specificity, and quantification capabilities. Other methods include Northern blotting, which is useful for assessing mRNA size and quantity, and RNA sequencing (RNA-seq), which provides a comprehensive view of the transcriptome, including enzyme mRNA expression levels.

Q: How can one ensure the specificity of enzyme mRNA to its target protein?

A: Ensuring specificity involves careful design of the mRNA sequence, including the use of specific codons that match the desired protein, and incorporating untranslated regions (UTRs) that can affect translation efficiency and mRNA stability. Additionally, employing sequence verification techniques, such as Sanger sequencing, after synthesis can confirm the accuracy of the mRNA sequence.

Q: What role do 5' and 3' untranslated regions (UTRs) play in the expression of enzyme mRNA?

A: The 5' and 3' UTRs play crucial roles in regulating mRNA stability, localization, and translation efficiency. The 5' UTR affects the initiation of translation, while the 3' UTR can influence mRNA stability and the efficiency of translation through elements that bind to specific proteins and microRNAs.

Q: What factors influence the degradation rate of enzyme mRNA in cells?

A: The degradation rate can be influenced by sequences within the mRNA, such as AU-rich elements (AREs) in the 3' UTR, which can target the mRNA for rapid degradation. Other factors include interactions with RNA-binding proteins and microRNAs, which can either stabilize the mRNA or target it for degradation.

Q: How do you design mRNA sequences for efficient expression of the enzyme?

A: Designing mRNA sequences for efficient enzyme expression involves optimizing the codon usage to match the host's preferred codon usage, ensuring efficient translation. Additionally, incorporating untranslated regions (UTRs) that stabilize the mRNA, and adding a 5' cap and a poly(A) tail are crucial for stability and efficient translation. Software tools and databases can help predict optimal codon usage and mRNA stability factors for specific host organisms.

Q: What considerations must be taken into account for efficient transfection of enzyme mRNA into cells?

A: For efficient transfection, the choice of transfection method (lipid-based, electroporation, or nanoparticle-based) should be compatible with the cell type. It's crucial to optimize the mRNA concentration, the ratio of mRNA to transfection reagent, and the duration of transfection to maximize uptake while minimizing cytotoxicity. Additionally, the presence of serum and antibiotics in the culture medium can affect transfection efficiency and should be optimized accordingly.

Q: How can the stability of enzyme mRNA be enhanced for longer expression durations?

A: The stability of enzyme mRNA can be enhanced by incorporating modified nucleotides (such as pseudouridine or 5-methylcytidine), optimizing the sequence of the 5' and 3' UTRs to enhance binding of stabilizing proteins, adding longer poly(A) tails, and using mRNA circularization techniques. These modifications can significantly increase the half-life of mRNA in cells, leading to prolonged expression.

IVTScrip™ mRNA-Human Intestinal Alkaline Phosphatase, (Cap 0, Pseudo-UTP, 120 nt-poly(A)) (CAT#: GTTS-WK0001MR)

Product type mRNA

Transcript Intestinal Alkaline Phosphatase

Category (CDS) Enzyme

Applications Gene therapy research