Small Angle X-Ray Scattering

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With advanced equipment and experienced experts, Creative Biolabs is an innovator and problem solver in the mRNA delivery industry. We can provide custom solutions for our clients in the implementation of projects based on small angle X-ray scattering (SAXS). Here are some examples of SAXS technology applications.

SAXS Characterization of Solid Lipid Nanoparticles (SLNs) and Nanostructured Lipid Carriers for Dibucaine (DBC) Encapsulation

This study aimed to characterize SLNs and NLCs prepared from cetyl palmitate or myristyl myristate, a mixture of capric and caprylic acids plus Pluronic F68 for encapsulation of DBC. A multiple-phase system such as SLNs and NLCs, with different densities of electrons and nanometric dimensions, can be dissected by X-ray scattering techniques, which provides important information as to their molecular organization. The peaks observed in SAXS diffractograms are associated with the presence of ordered phases, such as liquid crystals. From the peak position, one can calculate the repeat distances and finally obtain the frame type of the system. Consequently, the use of the SAXS aimed at a better understanding of the structural organization of the lipids that form the core of SLNs and NLCs. Fig.1A shows the scattering intensity curve, as a function of the scattering q vector, for the SLNCPDBC sample. Two peaks could be observed at 1.61 and 1.44nm-1 with repeating intervals. These peaks are characteristic of the CP nanoparticles and therefore represent the specific arrangements of the CP lipids within the nanoparticles. Moreover, they have the bilayer core-to-bilayer core distances of 38.9Å (lamellar phase I) and 43.5Å (lamellar phase II). The result shows that at fixed temperatures, below 30oC, structural changes were observed. However, above this temperature, just small changes were visualized, but without compromising the internal structure of the particle. There were no changes in the SAXS diffractograms of SLNs measured in the absence or the presence of DBC. SAXS results indicated the existence of lamellar lipid arrangements inside both SLNs and NLCs.

SAXS: scattering intensity vs q vector in (A) SLNCPDBC, measured at increasing (20, 30, and 40 oC) temperatures, and (B) in an SLNCP sample, with and without DBC, measured at 20 oC.Fig.1 SAXS: scattering intensity vs q vector in (A) SLNCPDBC, measured at increasing (20, 30, and 40oC) temperatures, and (B) in an SLNCP sample, with and without DBC, measured at 20 oC. (Barbosa, 2018)

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Reference

  1. Barbosa, R.M.; et al. Electron paramagnetic resonance and small-angle X-ray scattering characterization of solid lipid nanoparticles and nanostructured lipid carriers for dibucaine encapsulation. Langmuir. 2018, 34(44): 13296-13304.
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