With advanced equipment and experienced experts, Creative Biolabs is an innovator and problem solver in the mRNA delivery industry. We can provide custom solutions for our clients in the implementation of projects based on small angle X-ray scattering (SAXS). Here are some examples of SAXS technology applications.
This study aimed to characterize SLNs and NLCs prepared from cetyl palmitate or myristyl myristate, a mixture of capric and caprylic acids plus Pluronic F68 for encapsulation of DBC. A multiple-phase system such as SLNs and NLCs, with different densities of electrons and nanometric dimensions, can be dissected by X-ray scattering techniques, which provides important information as to their molecular organization. The peaks observed in SAXS diffractograms are associated with the presence of ordered phases, such as liquid crystals. From the peak position, one can calculate the repeat distances and finally obtain the frame type of the system. Consequently, the use of the SAXS aimed at a better understanding of the structural organization of the lipids that form the core of SLNs and NLCs. Fig.1A shows the scattering intensity curve, as a function of the scattering q vector, for the SLNCPDBC sample. Two peaks could be observed at 1.61 and 1.44nm-1 with repeating intervals. These peaks are characteristic of the CP nanoparticles and therefore represent the specific arrangements of the CP lipids within the nanoparticles. Moreover, they have the bilayer core-to-bilayer core distances of 38.9Å (lamellar phase I) and 43.5Å (lamellar phase II). It was shown that structural changes can be observed at fixed temperatures below 30℃. Above this temperature, only minor changes are seen, but do not affect the internal structure of the particles. There were no changes in the SAXS diffractograms of SLNs measured in the absence or the presence of DBC. SAXS results indicated the existence of lamellar lipid arrangements inside both SLNs and NLCs.
Fig.1 SAXS: scattering intensity vs q vector in (A) SLNCPDBC, measured at increasing (20, 30, and 40oC) temperatures, and (B) in an SLNCP sample, with and without DBC, measured at 20 oC. (Barbosa, 2018)
Creative Biolabs is a world-leading biotechnology company. We offer well-established and innovative one-stop-shop mRNA delivery solutions. Our strategic consultants and technical team are the most renowned experts in their fields. We are flexible to meet the unique needs of mRNA client projects. You can count on our skilled and passionate workforce to find the most suitable solution. Please contact us for more information and a detailed quote.
Reference
