Signal Transduction mRNA

Introduction

Signal transduction molecules interact to convert and transmit signals constituting various signal transduction pathways. Cells can respond to environment with relevant effects through signalling pathways, ultimately regulating specific gene expression, metabolism and apoptotic processes. The signalling process involves a range of important functional proteins, including extracellular signalling molecules (such as hormones), membrane proteins, receptors and enzymes. When any part involved goes wrong, the body is likely to develop related diseases. Creative Biolabs offers a wide range of signal transduction mRNA products carefully designed and optimised by an established and dedicated team of experts to encode the most common signal transduction proteins such as synaptophysin, SOST, Go-alpha, and CD series. We also provide you with nucleoside modification options (e.g. Pseudo-UTP, N1-Methylpseudo-UTP), so that you can choose appropriate product according to your experimental project. Signal transduction mRNA products are mainly used in the study of signal transduction pathway mechanism, related disease mechanism investigation and gene therapy method research.

For the flexibility of our IVTScrip™ synthesis platform, our experts can also provide additional customisation options according to your project requirements within a very short delivery time. The customisation service for signal transduction mRNA products focuses on strand length, nucleoside modification type, poly(A) length and 5' cap end optimisation. We also ensure that the entire process is strictly QC compliant, with purification results and mass spectrometry characterisation provided at each step. If you are interested in our products, please feel free to contact us.

Product List

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Product Features

• Strict Quality Control Standards
  • Multiple quality control standards.
  • High stability and batch-to-batch consistency.
  • Excellent purity and stability profile.
• Efficient and Time-saving
  • Pre-designed capping and poly (A) tail modification.
  • Shorter turnaround time.

Application

• Cancer Research: In the realm of cancer research, signal transduction mRNA plays a pivotal role in understanding how alterations in signaling pathways contribute to tumorigenesis, tumor growth, and metastasis. As an example, research has shown that the expression levels of Receptor Tyrosine Kinases (RTKs) mRNA are upregulated in various types of cancer, resulting in the overactivation of cell proliferation signals. By utilizing quantitative PCR techniques to measure the expression levels of specific RTK mRNA in tumor tissue samples, researchers can identify potential targets for cancer treatment.
• Disease Diagnosis: Signal transduction mRNA is instrumental in developing diagnostic markers for early disease detection. As an example, in cardiac disease research, it has been found that myocardial cells change the mRNA expression of certain signaling molecules, such as cAMP-response element-binding protein (CREB), when they are damaged. By analyzing the level of CREB mRNA in blood samples, it can serve as an early marker for cardiac attacks.
• Drug Development: In drug development, targeting the mRNA of signal transduction molecules offers a strategy for identifying new therapeutic agents. For example, studies on the PI3K/Akt signaling pathway, implicated in cancer cell survival and growth, involved screening compounds that could downregulate Akt mRNA expression in cancer cell lines. Through high-throughput screening and subsequent mRNA level quantification via qPCR, researchers identified novel small-molecule inhibitors of Akt. This exemplifies how manipulating signal transduction mRNA can aid in discovering new drugs with potential cancer therapy applications.
• Immune Regulation: Signal transduction mRNA is crucial for regulating the immune response, including T cell activation and differentiation. For example, a notable study focused on the T cell receptor (TCR) signaling pathway, specifically analyzing ZAP-70 mRNA expression, a kinase critical for T cell signaling. By quantifying ZAP-70 mRNA levels in T cells from patients with autoimmune diseases versus healthy controls, researchers could link aberrant ZAP-70 expression to autoimmune pathogenesis to better understand the significance of signal transduction mRNA in modulating immune responses.
• Cell Death and Survival Research: The study of cell death and survival heavily relies on understanding the mRNA expression of signal transduction molecules involved in apoptotic pathways. For example, research into the mRNA levels of Bcl-2 family members, which regulate the intrinsic apoptosis pathway, has provided insights into the mechanisms of programmed cell death. One study employed RT-PCR to measure the mRNA levels of pro-apoptotic (e.g., Bax) and anti-apoptotic (e.g., Bcl-2) genes in response to chemotherapeutic agents in cancer cells. The balance between these mRNA levels determines the cells' fate, highlighting how signal transduction mRNA research contributes to our understanding of cell survival mechanisms and the development of more effective cancer treatments.

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