Reporter Gene mRNA Products

Introduction Product List Product Features Application Q&A

Introduction

Reporter genes are genes encoding fluorescent proteins that enable the detection of gene expression, which can therefore be used as controls in various types of assay. Creative Biolabs provides well-designed reporter mRNAs that can be readily applied in mRNA-related researches for measuring transfection both in vitro and in vivo. Besides the part of CDS, we offer various options on each one reporter mRNA transcript in terms of the 5'-Capping (e.g. Cap0, Cap1, ARCA, etc.), 3'-tailing (e.g. 30 nt-polyA, 120 nt-polyA, etc.), modifications for single bases (e.g. 5-Methoxy-U, Cyanine 5-U, etc.).

Besides the predesigned reporter mRNA products, custom IVT mRNA synthesis is also available at Creative Biolabs for reporter genes with specific features which are not in our increasingly developed inventory of mRNA transcript products. The custom synthesis can be performed using the template DNA either from clients or in-house at Creative Biolabs. If you are interested in our products, please feel free to contact us.

Product List

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Product Features

Strict Quality Control Standards
- Multiple quality control standards.
- High stability and batch-to-batch consistency.
- Excellent purity and stability profile.
Efficient and Time-saving
- Pre-designed capping and poly (A) tail modification.
- Shorter turnaround time.

Application

Gene Expression Studies: Scientists use reporter genes to indicate if a particular gene has been taken up and expressed in the cell, allowing for easy and rapid quantification of gene expression.
Cell Tracing Experiments: Cells can be marked with a reporter gene, allowing researchers to track and locate them within a larger population of cells, or even within a living organism.
Drug Discovery and Development: Reporter gene assays are frequently used in high-throughput screening for drug discovery. They provide a quick and convenient way of sorting out compounds that affect gene expression.
Protein-Protein Interaction Studies: The reporter gene can be used in fusion with a protein of interest to study protein-protein interaction.
Gene therapy development: Reporter genes can be used to monitor the presence and expression of therapeutic genes.
Studying biological processes: They can be used to study different biological processes like cell differentiation, cell division, cell to cell communication and understanding cellular responses to environmental conditions.
Promoter analysis: If a reporter gene is downstream of a promoter, the reporter gene's expression can be used to determine the activity of the promoter.

Q&A

Q: How effective is report gene mRNA in tracking gene expression?

A: Report gene mRNA is very effective for tracking and quantifying gene expression. It allows for real-time monitoring, giving researchers immediate feedback on the effectiveness of a treatment or manipulation.

Q: Can report gene mRNA be used in all cell types?

A: Most cell types are receptive to mRNA transfection, but efficiency can vary. It's recommended that researchers optimize conditions for their specific cell type.

Q: How stable is report gene mRNA?

A: Report gene mRNA is not as stable as DNA, which could affect long-term studies. However its instability can be an advantage for short term studies because it allows for transient expression of the target gene.

Q: Can report gene mRNA be used in vivo, or is it only suitable for in vitro studies?

A: Report gene mRNA can be used both in vivo and in vitro. However, it might require different delivery mechanisms. In vitro, simple transfection is usually sufficient, while in vivo often requires complex delivery mechanisms.

Q: Can using a reporter gene mRNA influence the expression of the target gene?

A: In most cases, the expression of the reporter gene does not affect the expression of the target gene. However, in certain situations, interference can occur. It's crucial to validate results with negative controls and other experimental methods.

Q: How long does the expression from reporter gene mRNA last?

A: The expression from reporter gene mRNA is transient, often peaking within 24-48 hours post-transfection. The actual duration can depend on factors like cell type, mRNA stability, and the particular reporter gene used.

Q: Can multiple reporter genes be used simultaneously?

A: Yes, multiple reporter genes can be co-transfected simultaneously. This can be useful for internal normalization or to study multiple genes or pathways within the same sample.

Q: What challenges may arise in detecting the signal from the reporter gene mRNA?

A: Difficulty in detecting reporter gene signals can stem from a variety of sources, including insufficient transfection efficiency, low reporter gene expression, or issues with the detection method. Enhancing transfection efficiency, increasing the sensitivity of the detection system, or changing the reporter gene used might resolve these issues.