LncRNA (long non-coding RNA)

Introduction

Long non-coding RNAs (LncRNAs) are a class of non-protein-coding RNAs longer than 200 nt that have important regulatory functions in transcriptional silencing, transcriptional activation, chromosomal modification and intranuclear transport. LncRNAs are mainly classified as antisense IncRNA, intronic transcript, large intergenic noncoding RNA, promoter-associated IncRNA and UTR associated InGRNA. Creative Biolabs provides you with a list of commonly used LncRNAs including AK057596, AK093551, AK095700, AK124399, AK125727, AK307192, AL117190.2, ANP32A-IT1, and AP4B1-AS1. Furthermore, these pre-made LncRNAs can also be categorised according to different nucleoside modifications (e.g. pseudo-UTP, N1-methyl pseudo-UTP, 2-thio-UTP). As the most specific non-coding protein RNA, LncRNAs are mainly applied to study the biological regulation mechanisms of LncRNAs in development and disease, which are also important for the discovery of related signalling pathways and gene therapy drugs.

Besides, with a team of experienced experts, Creative Biolabs can provide you with customisation services for specific long-stranded non-coding RNAs. According to your project requirements, our one-stop customisation service for long-stranded non-coding RNAs can give you customisable options including template design, strand length, nucleotide modification type, and poly(A) plus tail length. QC standards are followed throughout entire customisation process to ensure product quality. Moreover, each step is accompanied by purification and mass spectrometry analysis to ensure the purity and intended design of the customised LncRNAs. With our unique IVTScrip™ mRNA synthesis platform, both pre-made and custom RNAs are available at competitive prices and within short delivery time. If you are interested in our products, please feel free to contact us.

Product List

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Product Features

• Strict Quality Control Standards
  • Multiple quality control standards.
  • High stability and batch-to-batch consistency.
  • Excellent purity and stability profile.
• Efficient and Time-saving
  • Pre-designed capping and poly (A) tail modification.
  • Shorter turnaround time.

Application

• Gene Expression Regulation: LncRNAs can regulate gene expression at various levels, including transcriptional and post-transcriptional regulation. One well-known example is the lncRNA XIST, which is essential for X-chromosome inactivation in female mammals. XIST coats the X chromosome from which it is transcribed, recruiting chromatin-modifying complexes to silence gene expression. Studies on XIST have shed light on the mechanisms of epigenetic gene regulation and the importance of lncRNAs in development and disease.
• Chromatin Remodeling and Organization: LncRNAs play significant roles in chromatin remodeling and the organization of the genome within the nucleus. The lncRNA HOTAIR, for instance, reprograms chromatin state to promote cancer metastasis. HOTAIR interacts with the Polycomb Repressive Complex 2 (PRC2) and guides it to specific genomic loci to epigenetically repress gene expression. Research on HOTAIR has contributed to our understanding of the epigenetic basis of cancer and highlighted the potential of lncRNAs as therapeutic targets.
• Cellular Differentiation and Development: LncRNAs are key regulators of cellular differentiation and development. The lncRNA FENDRR is critical for heart and body wall development in the mouse. FENDRR binds to both PRC2 and the Trithorax group (TrxG) chromatin-modifying complexes, acting as a scaffold to regulate the expression of genes involved in development. Studies of FENDRR and other lncRNAs have elucidated their roles in cell fate determination and organogenesis.
• Disease Mechanism Studies: LncRNAs are implicated in a variety of diseases, including cancers, cardiovascular diseases, and neurological disorders. MALAT1 (Metastasis-Associated Lung Adenocarcinoma Transcript 1) is a lncRNA that is upregulated in several types of cancer and is associated with metastasis and poor prognosis. Research on MALAT1 has focused on understanding its role in cancer progression and exploring its potential as a biomarker for cancer diagnosis and a target for therapy.
• Immune Response Regulation: LncRNAs are involved in the regulation of the immune response. For example, the lncRNA NEAT1 is essential for the formation of paraspeckles, nuclear bodies involved in the regulation of gene expression in response to stress. NEAT1 has been shown to regulate the expression of genes involved in innate immunity, influencing the response to viral infections. Studies on NEAT1 highlight the importance of lncRNAs in the regulation of the immune system and their potential as targets for treating infectious diseases.
• Bioinformatics and Systems Biology: With the advent of high-throughput sequencing technologies, bioinformatics and systems biology approaches have become essential for identifying and characterizing lncRNAs and their functions. These approaches have enabled the discovery of thousands of lncRNAs and the analysis of their expression patterns, molecular interactions, and functional annotations in various biological contexts. Integrative studies have provided insights into the complex regulatory networks involving lncRNAs, revealing their roles in health and disease.

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