Visualizing RNA at Nucleus-Resolved Level
Gain Direct Measurement of Endogenous RNA in Native Complexes in Non-Clinical Settings
Creative Biolabs Nuclease Protection Assay (NPA) offers high precision in RNA expression profiling, RNA stability and RNA subcellular localization analysis for non-clinical settings. This platform preserves endogenous RNA molecules in intact nuclei or subcellular structures, then uses site-specific nuclease digestion to remove unprotected RNA fragments. Thus, NPA is highly beneficial for epigenetics, virology and cancer studies that require elimination of amplification bias, detection of low-abundance RNA targets, and reconstruction of RNA regulation networks with minimal disturbance of native complexes.
In contrast to amplification-dependent approaches (RT-qPCR, RNA-Seq), NPA allows for the direct quantification of RNA through:
Preservation of Native Complexes
Subcellular Location Resolution
Absolute Quantification
| Conventional Methods | Our Approach | Research Advantage |
|---|---|---|
| Amplification-dependent detection | Direct probe hybridization | Avoids enzymatic artifacts |
| Indirect stability inference | Time-resolved enzymatic interrogation | Captures dynamic processes |
| Limited spatial context | Subcellular compartment compatibility | Resolves localization mechanisms |
Our Nuclease Protection Analysis provides researchers with a specialized methodology to interrogate RNA within its endogenous molecular environment. This service employs targeted enzymatic strategies to maintain RNA-protein complexes in their native state, enabling direct investigation of RNA stability dynamics and spatial organization.
Fig.1 Workflow of Nuclease Protection Analysis Service.
Time-resolved enzymatic digestion provides a molecular level demonstration of RNA stability under a defined set of conditions. This assay can be used to monitor the kinetics of RNA degradation upon perturbation. This is useful for studying the life cycle of virus infection, post-transcriptional regulatory mechanisms and to screen putative drug targets. A crosslinking-free buffer is used to preserve RNA-protein complexes in their native state during the assay.
Sense vs. antisense-discriminating probe design is used to identify strand-specific binding partners from complex biological samples. This service can be used to study non-coding RNA function, virus replication intermediates and transcriptional interference with high confidence, and includes bioinformatics to identify and design probes that will not cross-hybridize to unintended targets.
Subcellular localization of RNA is achieved through fractionation-compatible sample processing. This service is used for mapping RNA localization within different subcellular compartments. Analysis can be applied to study nucleocytoplasmic transport, organelle specific RNA function, and other localization dependent regulatory mechanisms in model systems.
Leveraging Enzymatic Protection Principles for Mechanistic Discovery
Technology Value: Directly quantifies transient viral RNA intermediates often masked by host RNA background, enabling real-time tracking of replication cycles without amplification artifacts.
Technology Value: Captures rapid turnover of low-abundance regulatory RNAs that drive cellular reprogramming, preserving native structure-protein interactions.
Technology Value: Maintains spatial fidelity during fractionation to resolve RNA localization defects in subcellular compartments below organelle-scale resolution.
Unparalleled Methodological Fidelity
We deliver comprehensive analysis of RNA molecular dynamics within native architectures – from viral replication studies to subcellular localization mechanisms – exclusively for non-clinical research. Our platform integrates with multi-omics workflows (RNA-Seq, proteomics) to reveal regulatory insights inaccessible through fragmented approaches.
Unified Technology Ecosystem
Cross-Method Synergy: Combine with PrimeFlow for spatial validation or RNA methylation profiling for modification context
Dynamic Process Capture: Time-resolved enzymatic interrogation tracks RNA decay trajectories in live systems
Architectural Preservation: Fractionation-compatible protocols maintain compartment-specific interactions
Dedicated Scientific Partnership
PhD-Level Experimental Design: Domain experts (5+ years RNA biochemistry) develop species-optimized strategies
Mechanistic Framing Workshops: Collaborative data interpretation through disease mechanism lenses
Funding Grant Support: Technical figure generation for NIH/NSFC/ERC proposals
Rigorous Quality Commitment
Tiered QC Protocol: Three-stage verification from hybridization to fragment analysis
Orthogonal Confirmation: Key findings cross-validated via Northern blot/RNA-Seq
Process Transparency: Full raw data access with analytical parameter documentation
Share target RNA(s), biological model, and core questions. Our specialists provide:
[Contact Us] Schedule a technical consultation within 24 hrs.
A: Research-grade cellular lysates or tissue homogenates from model organisms.
A: Project-specific schedules are provided after sample quality assessment.
A: Custom integrated packages are available through project consultation.
A: All services incorporate process controls and replication verification.
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