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Custom mRNA Modification

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
Background mRNA Modification Highlights FAQs Published Data


As a leading biotechnology company in the world, Creative Biolabs offers a full package of custom mRNA modification services to promote the development of mRNA therapeutics. With the help of our highly experienced staff, we can improve the pharmacokinetic properties of custom mRNA both in terms of stability and decreased immunogenicity. Our goal is to provide you with the most affordable, high-quality custom mRNA modification services to ensure your satisfaction in a timely and professional manner.

  • Introduction of Synthetic mRNA Modification

During the past years, mRNA-based therapy which is founded on the induction of the transient translation with the cell's ribosomes of fully functional proteins without integration into the host genome has become a potential drug for cancer immunotherapy and prophylactic vaccines. Compared with conventional gene therapies and protein substitution strategies, in vitro synthetic (IVT) mRNA has several advantages. However, caused by the lack of a cap structure at the 5' end and a long sequence of polyadenylate residue (poly(A) tail) at the 3' end hamper the development of mRNA therapeutics for many years. As the cap structure and the poly(A) tail of the mRNA greatly enhance the stability of natural mRNA, synthetically produced mRNA can also be modified to contain these structures for optimization. Using these modifications, the stability of synthetic mRNAs can be improved, immunogenicity can be reduced, and the translation efficiency can be increased.

Diagram of IVT mRNA Structure.

Fig.1 Schematic structure of in vitro transcribed mRNA. 1

mRNA Modification Services

The application of IVT mRNA is a versatile and promising tool for the delivery of transgenes into desired cells. To promote the development of mRNA-based therapeutics, Creative Biolabs has established an advanced mRNA development platform. With abundant experience and professional scientists, we are capable of both custom mRNA synthesis and custom mRNA modification services for global researchers. Particularly, the modification of mRNA products can be accomplished by either incorporation of modified nucleosides at the 3' end or a cap structure at the 5' end. Our scientists will select the most appropriate modification approach to minimize the risks of mutagen and carcinogen effects of your mRNA product.

Inquire About Our Services


  • Customized modification options to meet each clients' needs
  • Flexible modification approaches available
  • Professional scientists and technicians with extensive experience
  • Short turn-around time with the best after-sale service


Q: What is the purpose of mRNA modification?

A: mRNA modification improves the stability, reduces immunogenicity, and increases the translation efficiency of synthetic mRNAs.

Q: What types of modifications are available for mRNA at Creative Biolabs?

A: The modified service provided by Creative Biolabs includes, but is not limited to, adding a synthetic cap at the 5 'end, a poly (A) tail at the 3' end, and a modified nucleoside, such as pseudouridine or 5-methylcytidine.

Q: How does Creative Biolabs ensure the quality of mRNA modifications?

A: Our experienced scientists use advanced technology to customize and optimize modifications to ensure high quality and stable mRNA products.

Q: Can modifications be tailored to specific needs?

A: Yes, Creative Biolabs provides customized modification options to meet the specific needs of each customer.

Q: What are the benefits of mRNA modifications provided by Creative Biolabs?

A: The benefits include enhanced stability, reduced immunogenicity, and improved translational activity of synthetic mRNAs.

Q: How can clients get more information about the services?

A: Clients can contact us directly for more detailed information and to discuss specific project requirements.

Published Data

  • mRNA Modification

This article investigates how the mRNA modification 5-methylcytosine (m5C) influences rice's response to heat stress. The study identifies OsNSUN2 as a key mRNA m5C methyltransferase in rice, crucial for maintaining photosynthesis and chloroplast function under high temperatures. OsNSUN2 mutations lead to hypersensitivity to heat, impaired photosynthesis, and excessive reactive oxygen species (ROS) accumulation. The research suggests that OsNSUN2-dependent m5C modification enhances the translation of stress-responsive mRNAs, aiding rice adaptation to heat stress. Creative Biolabs offers flexible custom mRNA services to help our clients overcome challenges encountered in mRNA research.

Analysis results of osnsun2-dependent mRNA m5c modification.

Fig.1 Analysis and detection of osnsun2-dependent mRNA m5c modification. 2


  1. Steinle, H.; et al. Concise review: application of in vitro transcribed messenger RNA for cellular engineering and reprogramming: progress and challenges. Stem Cells. 2017, 35(1): pp.68-79.
  2. Tang, Yongyan, et al. "OsNSUN2-mediated 5-methylcytosine mRNA modification enhances rice adaptation to high temperature." Developmental cell 53.3 (2020): 272-286.
All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.