Custom mRNA chemical synthesis is an advanced non-enzymatic method with better control and precision than traditional enzymatic approaches. It enables atomic-level sequence control and site-specific chemical modifications, addressing immunogenicity/stability issues to speed research-to-clinical development—critical for high-purity, effective mRNA for vaccines and non-coding immunotherapies.
Creative Biolabs' corresponding service solves challenges like high-fidelity transcripts and IVT limitations via an advanced solid-phase platform, producing pure, consistent, stable mRNA with atomic-level fidelity and site-specific modifications for diverse uses.
Discover How We Can Help - Request a ConsultationThis is the most common form of synthesis, where the mRNA molecule is built as a single, linear strand from 5' to 3' on a solid support. This method is highly flexible and allows for the synthesis of transcripts of varying lengths with precise sequence control and the ability to incorporate modifications at any position.
Fig.1 Schematic diagram of the solid-phase synthesis method of RNA.1,3
While less common than linear, circular mRNA (circRNA) is a growing field of interest due to its enhanced stability and long-lasting protein expression. CircRNA lacks free ends, making it resistant to degradation by exonucleases. Our service is capable of producing circRNA via advanced ligation techniques, offering a robust platform for long-term expression applications.
Fig.2 The existing methods for synthesizing circular RNA.2,3
Our primary method is solid-phase chemical synthesis using phosphoramidite chemistry. This allows for a stepwise, controlled addition of each nucleotide to the growing chain. This approach provides several key advantages: high purity of the final product, the ability to incorporate a diverse range of chemical modifications, and precise control over the overall sequence length and integrity.
Upon completion, you receive purified mRNA plus a detailed Certificate of Analysis (CoA) with full QC report, synthesis summary, and data for regulatory filings.
Typical 4-8 weeks, depending on mRNA length, modification complexity, and specific QC needs. We provide a precise timeline after a thorough review.
One-stop solution
One-stop solution for Custom mRNA Chemical Synthesis, guiding you from initial design to large-scale, GMP-grade production.
Advanced phosphoramidite chemistry
Advanced phosphoramidite chemistry for superior sequence fidelity and the precise, site-specific incorporation of a wide range of modified nucleotides.
Exceptional scalability
Exceptional scalability to meet your needs, from small-scale discovery research to multi-gram quantities for preclinical and clinical development.
Full documentation and transparency
Full documentation and transparency to support regulatory submissions, adhering to Good Manufacturing Practice (GMP) principles.
Superior Purity
Our multi-stage purification process, including HPLC, effectively removes impurities and byproducts such as double-stranded RNA (dsRNA), which can trigger unwanted innate immune responses. Published data confirms our ability to deliver ultra-pure mRNA.
Reliable and Reproducible
Our standardized, validated processes ensure lot-to-lot consistency and scalability, offering a reliable pathway from early-stage research to GMP-grade manufacturing.
A: While IVT is a useful tool for initial research, it is an enzymatic process prone to impurities and sequence errors. Chemical synthesis, in contrast, offers a defined, atomic-level control over the sequence and allows for the precise incorporation of modifications, which is crucial for reducing immunogenicity and enhancing stability for therapeutic applications.
A: Absolutely. We offer expert consultation services to assist with sequence optimization, including codon optimization for enhanced protein expression and the design of UTRs that can improve mRNA stability and translational efficiency. Our team is here to help you get the best results from your project.
A: We employ a multi-step purification process, including advanced HPLC, to ensure high purity. Every batch is subjected to rigorous quality control testing, including mass spectrometry and gel electrophoresis, and we provide a detailed Certificate of Analysis so you can have complete confidence in your materials.
A: We can incorporate a wide variety of modified nucleosides to meet your specific needs. Common examples include N1-methylpseudouridine (m1ψ), pseudouridine (ψ), and 5-methylcytidine (m5C), all of which are critical for enhancing stability and reducing the innate immune response.
A: Our chemical synthesis platform is highly scalable, and we have established manufacturing processes that can bridge the gap from research-grade quantities to GMP-grade material required for clinical trials. Our consistent and well-documented workflow simplifies your regulatory process and ensures reliability at every stage.
To learn more about our offerings and to discuss how our Custom mRNA Chemical Synthesis service can be tailored to your specific project needs, please do not hesitate to reach out. Our team of experts is ready to provide you with detailed information, a customized proposal, and technical guidance to accelerate your research and development efforts.
Contact Our Team for More Information and to Discuss Your Project| Cat. No | Product Name | Promoter |
|---|---|---|
| CAT#: GTVCR-WQ001MR | IVTScrip™ pT7-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ002MR | IVTScrip™ pT7-VEE-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ003MR | IVTScrip™ pT7-VEE-mRNA-FLuc Vector | T7 |
| CAT#: GTVCR-WQ87MR | IVTScrip™ pT7-VEE-mRNA-Anti-SELP, 42-89-glycoprotein Vector | T7 |
| Cat. No | Product Name | Type |
|---|---|---|
| CAT#: GTTS-WQ001MR) | IVTScrip™ mRNA-EGFP (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| CAT#: GTTS-WK18036MR | IVTScrip™ mRNA-Human AIMP2, (Cap 1, Pseudo-UTP, 120 nt-poly(A)) | Enzyme mRNA |
| (CAT#: GTTS-WQ004MR) | IVTScrip™ mRNA-Fluc (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| (CAT#: GTTS-WQ009MR) | IVTScrip™ mRNA-β gal (Cap 1, 30 nt-poly(A)) | Reporter Gene |
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