Electroporation of mRNA

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  • Electroporation of mRNA

    Electroporation uses a short high-voltage pulse to create temporary pores in cell membranes through which mRNA and other nucleic acids can enter into a variety of cell types. This technique is an easy, inexpensive, highly efficient, and scalable method, used as a versatile mRNA delivery method for a wide range of prokaryotes and eukaryotes. Creative Biolabs has accumulated rich experience in the field of electroporation-based mRNA transfection and provides the best and affordable mRNA electroporation services to accelerate your project success.

    Introduction of Electroporation

    Electroporation is a common and effective transfection method that enables some substances such as chemicals, RNA, and DNA to transfer into a variety of different cell types under electrical impulses. This method is easy, flexible, highly efficient, inexpensive, and scalable, so it has been widely applied in the field of RNA research. Electroporation mRNA into human cells provides a powerful platform to transiently express proteins of choice in a variety of different cell types, such as dendritic cells (DCs), T cells, B cells, bulk peripheral blood mononuclear cells (PBMCs), primary cells, embryonic cells. Electroporation is even applied to introduce mRNA to whole tissues in vivo. However, there have some unavoidable drawbacks of electroporation transfection, such as substantial cell death caused by high voltage pulses. It is possible to increase transfection efficiency and reduce cell death by optimizing electroporation conditions such as voltage.

    Optimized mRNA electroporation procedure results in uniform high-level surface expression Fig.1 Optimized mRNA electroporation procedure results in uniform high-level surface expression and specific function of CAR-T cells in vitro. (Barrett, 2011)

    Applications of mRNA Electroporation

    • Immunotherapy

    Optimized mRNA electroporation procedure results in uniform high-level surface expressionElectroporation of mRNA into immune cells has been used to modify or enhance immune responses, applied for the treatment of various diseases. mRNA electroporation is a common method to load dendritic cells with tumor-associated antigens. Besides, costimulatory molecules such as toll-like receptor (TLR) in DCs can also be introduced into DCs to promote the maturation and activation of these DCs. Furthermore, mRNA electroporation is applied to engineer T cells to express chimeric antigen receptors (CARs) or normal T-cell receptors (TCRs) to redirect them to effectively recognize and lyse tumor cells.

    • Stem Cell Research Stem Cell Research

    Stem cell transplantation is a promising therapeutic strategy for neurological disease or injury, diabetes, and myocardial infarction. mRNA electroporation is a highly efficient gene transfer technology to load stem cells with genes to control and/or direct their differentiation towards specialized cell types, which are used for the treatment of various diseases.

    Advantages

    • Simplicity and inexpensive
    • High transfection efficiency
    • Suitable for almost all cell types, even whole tissues
    • Scalability (transfect a large number of cells in a short time)
    • Rapid expression of protein
    • Transiency of the expression
    • Absence of an influence on cell phenotype

    Electroporation of mRNA provides a widely applicable technique to transiently express proteins of choice in a variety of different cell types. Due to several advantages over other transfection methods, this technique is broadly applied in many fields, such as immunotherapy, directed induction of stem cells, transient expression of proteins in a variety of different cell types, and RNA vaccines. If you are interested in our mRNA electroporation services, please feel free to contact us for more details.

    Reference

    1. Barrett, D. M.; et al. Treatment of advanced leukemia in mice with mRNA engineered T cells. Human Gene Therapy. 2011, 22(12): 1575-1586.
    For Research Use Only.