With years of experience and our advanced platform, Creative Biolabs has developed a variety of mRNA stability tests for different requirements, including the actinomycin D-based method, c-fos serum-inducible promoter system, and Tet-off regulatory promoter system. Our scientists are confident in offering the best service and the most qualified outcomes for our customers all over the world.
mRNA stability is the major control point in gene expression regulation. Changes in mRNA stability are intimately related to alterations in gene expression regulation. mRNA stability is highly regulated by the interactions between structural elements of an mRNA transcript and a plethora of specific RNA-binding proteins. The deregulated mRNA stability could result in the aberrant accumulation of mRNAs and the proteins they encode. Different cellular processes, such as cell cycle progression, cell differentiation, and embryonic development are modulated by changes in mRNA stability.
Fig.1 Main classes of methods for mRNA stability.1
Determination of mRNA half-life, a measure of mRNA stability, provides important insights into changes in gene expression and the underlying mechanisms that regulate transcriptional levels under different physiological conditions or developmental stages. Creative Biolabs provides direct or indirect measurements of decay rates of endogenous mRNAs performed in several ways for our customers, including but not limited to:
The mRNA stability within cells can be measured indirectly by analyzing the mRNA half-life following transcription inhibition, where changes in mRNA levels are assumed to reflect mRNA degradation. Creative Biolabs provides one of the simplest techniques of measuring mRNA stability by inhibiting transcription with transcription inhibitors and measuring the mRNA kinetics. Transcription inhibition using the actinomycin D-based method has been proved to be the most amenable to high-throughput analysis and has opened the door to dissecting mRNA decay transcriptome-wide. In this manner, you can quantitate the half-life of your mRNA of interest in a very efficient and precise way.
According to the different expected applications, mRNA stability assays may need to be custom designed and developed. Combined with our advanced techniques and over ten years of extensive experience, Creative Biolabs can provide assay development to satisfy various project demands.
Aside from the actinomycin D-based methods for measuring endogenous mRNA stability, Creative Biolabs can determine the mRNA stability using c-fos serum-inducible promoter system and Tet-off regulatory promoter system. These systems are performed by transiently transfecting the reporter gene into mammalian tissue culture cells and using a pulsing transcriptional approach to monitor deadenylation and decay kinetics of the reporter transcript. We can use two promoter systems for different applications to test half-lives of mRNAs of interest in mammalian tissue culture cells with transient transfection systems to meet our customers' desired project demands.
A: It measures the half-life and degradation rate of mRNA to understand gene expression dynamics under different conditions.
A: It influences gene expression levels and is crucial for understanding cellular responses, protein production, and the development of RNA-based therapeutics.
A: Techniques include actinomycin D treatment, c-fos serum-inducible promoter system, and Tet-off regulatory promoter system.
A: This method uses the c-fos promoter, which is activated by serum, to study mRNA stability by tracking the degradation of induced mRNA.
A: Creative Biolabs employs rigorous quality control protocols, including the use of internal standards and replicates, to ensure the reliability and accuracy of the test results.
A: Yes, Creative Biolabs offers custom assay development to meet specific research needs.
This article explores how the stability of mRNA is influenced by the process of translation initiation. The study uses non-invasive techniques to measure mRNA production and decay, revealing that translation initiation, rather than elongation or codon optimality, is the key factor determining mRNA stability in yeast. The findings show that mRNA degradation is closely linked to translation, with a competition between translation initiation and mRNA decay being the primary determinant of mRNA half-life. The study provides new insights into the dynamic nature of the transcriptome, highlighting the critical role of translation initiation in regulating mRNA stability and its implications for cellular function and gene expression.
Fig.2 mRNA stability measurements with metabolic labeling.2
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