Cell Marker mRNA

Introduction

Cellular markers are important markers used to define and sort out cells. Changes in the expression of certain specific molecules help to identify the presence of certain specific cells, and these molecules are generally proteins. Creative Biolabs offers a range of high quality cell marker mRNA products encoding markers for cancer cells (SBSN, MAGE-A4), stem cells (SSEA-4, STRO-1), red blood cells (TER119) and immune cells (myeloid inhibitory C-lectin). Under different cell marker mRNA product categories, you can also select the appropriate nucleotide modification (e.g. Pseudo-UTP, N1-Methylpseudo-UTP, 5-Methoxy-UTP) and 3'-tailing (e.g. 30 nt-polyA, 120 nt-polyA) of mRNAs. The cell marker mRNAs we offer are expertly designed and synthesised on the advanced IVTScrip™ platform, involving multi-step purification and mass spectrometry characterisation to ensure product authenticity. Cell marker mRNA is used in the development of cell marker assays, cell marker-related diseases and gene therapies.

Due to great variety of cellular markers in the organism, the pre-produced mRNAs we provide may only cover some commonly used cellular markers. However, our flexible and scalable IVTScrip™ technology platform can customise mRNAs for a variety of long strands and different modification strategies. Our experts have 10 years of experience in mRNA synthesis and can help you advance your programme as quickly as possible, saving time to complete development while ensuring stringent quality control. Placing an order in Creative Biolabs, you'll get more affordable prices and shorter turnaround time. If you are interested in our products, please feel free to contact us.

Product List

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Product Features

• Strict Quality Control Standards
  • Multiple quality control standards.
  • High stability and batch-to-batch consistency.
  • Excellent purity and stability profile.
• Efficient and Time-saving
  • Pre-designed capping and poly (A) tail modification.
  • Shorter turnaround time.

Application

• Identification and Isolation of Cell Populations: Cell marker mRNAs are widely used to identify and isolate specific cell types from a mixed population. This application is crucial in stem cell research, immunology, and cancer biology. For instance, the expression of the POU5F1 (Oct-4) mRNA serves as a marker for identifying pluripotent stem cells. Researchers have utilized fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS) techniques based on Oct-4 expression to isolate and study pluripotent stem cells, furthering our understanding of stem cell biology and potential therapeutic applications.
• Study of Cell Lineage and Differentiation: Tracking the expression of cell marker mRNAs over time allows researchers to study cell lineage and differentiation pathways. In developmental biology, the sequential expression of specific mRNAs, such as Brachyury (a marker for mesoderm formation) and Sox2 (a marker for neuroectoderm), has been used to map the lineage and differentiation pathways of embryonic stem cells into various tissue types. This research is fundamental for understanding organ development and the mechanisms underlying developmental disorders.
• Diagnosis of Diseases: Cell marker mRNAs are critical in diagnosing various diseases, including cancers and immune disorders. For example, the overexpression of the mRNA of the prostate-specific antigen (PSA) is a marker for prostate cancer. Quantitative RT-PCR assays targeting PSA mRNA are used in clinical settings to diagnose and monitor prostate cancer progression. Similarly, the expression levels of certain mRNAs can indicate the presence of specific leukemias or lymphomas, aiding in the accurate diagnosis and prognosis of hematological malignancies.
• Development of Targeted Therapies: Understanding the expression patterns of cell marker mRNAs in diseases has paved the way for the development of targeted therapies. For instance, in cancer research, the identification of mRNAs that are uniquely expressed in tumor cells but not in normal cells has led to the development of mRNA-based vaccines and therapies. An example includes the use of mRNA-based vaccines targeting melanoma-specific antigens, such as the mRNA for the differentiation antigen Melan-A/MART-1, providing a personalized approach to cancer immunotherapy.
• Bioinformatics and Systems Biology: Bioinformatics and systems biology approaches utilize cell marker mRNA expression data to construct cellular networks and pathways, offering insights into the complex regulatory mechanisms governing cell function and disease. By integrating mRNA expression data from various cell markers with other omics data, researchers can identify key regulatory nodes and pathways involved in cell differentiation, disease progression, and response to treatment. This comprehensive approach facilitates the identification of new therapeutic targets and the development of more effective treatments.

Q&A