Creative Biolabs is a trusted partner in mRNA services. With decades of experience in mRNA research and development, our scientists can provide both chemical synthesis approaches and IVT synthesis strategies of mRNA to meet the unique requirements of every client. Moreover, we can also offer quality control of mRNA products to advance your program and reduce the full discovery and development timeline.
Recently, synthesized mRNA has been developed for cell engineering, CRISPR/Cas9 genome editing, prophylactic vaccines, and therapeutics. Creative Biolabs is committed to providing customers with high-quality mRNA related services. Due to the rise of robust and reliable in vitro transcription (IVT) systems, mRNA can be generated at milligram to multigram scales with lengths ranging from a few hundred nucleotides to greater than 10 kilobases. Additionally, we can provide a variety of purification options and modification strategies for your clinical needs, and our suite of analytical services allows you to characterize mRNA thoroughly.
When the fragment of mRNA you need is small, we recommend using a chemical synthesis strategy, which is a quick and more economical method to produce a high yield of chemically synthesized mRNA for various applications. mRNAs containing nearly any sequences can be chemically synthesized and modified, and gram quantity yields are possible.
Starting with a DNA template you provide, or with our gene synthesis and template construction, Creative Biolabs produces high-quality long fragments of mRNA by the IVT method for a wide range of uses, from basic research to clinical applications. Our standard process delivers high-quality materials for research use, and we provide scale-up process development and custom manufacturing to meet your specific requirements for clinical use.
A comprehensive quality control program at Creative Biolabs can ensure mRNA purity and mass spectral analysis. We could confirm the veracity of the manufacturing process and an exceptional and consistent batch-to-batch quality as well as compliance with regulatory requirements. We can also provide additional custom options available per your project requirements.
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A: Creative Biolabs provides both chemical synthesis and in vitro transcription (IVT) synthesis of mRNA. These methods allow for the production of high-quality mRNA for a variety of applications, including research and clinical use.
A: We can provide custom synthesis in various specifications based on our clients' research needs. We are capable of producing mRNA from milligram to multi-gram scales, with lengths ranging from several hundred nucleotides to over 10,000 bases, fully meeting most research requirements. If you have specific needs, please contact us for detailed discussions.
A: We have a comprehensive quality control program that includes ensuring mRNA purity, mass spectral analysis, and consistent batch-to-batch quality. They also comply with regulatory requirements.
A: Creative Biolabs offers a variety of modification and purification options, including but not limited to capping services and nucleotide modification services. We also provide additional services for sequence addition and design. We strive to meet all of our clients' needs.
A: Synthesized mRNA can be used for cell engineering, CRISPR/Cas9 genome editing, prophylactic vaccines, and various therapeutics, among other applications.
This article details a method to produce synthetic mRNA with reduced immunogenicity using high-temperature in vitro transcription (IVT) with thermostable T7 RNA polymerases. The study identifies two types of double-stranded RNA (dsRNA) by-products that trigger immune responses: one from 3'-extension of run-off products and another from antisense RNA formation. The researchers demonstrate that high-temperature IVT reduces 3'-extension without affecting antisense RNA by-products. Additionally, incorporating a poly(A) tail in the template DNA decreases antisense RNA formation. This approach simplifies mRNA synthesis, potentially eliminating the need for post-synthesis purification to remove immunogenic dsRNA. Creative Biolabs has established a specialized technology platform for mRNA synthesis, which can address various issues in mRNA synthesis.
Fig.1 Agarose gel analysis results of 3'-extended RNA.1
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