Creative Biolabs is one of the well-recognized experts who are professionals in offering high-quality mRNA-based assays. According to the different goals and expected applications, different assays may need to be designed and prepared. With our rich experience in mRNA-based assay technology, Creative Biolabs is now offering a variety of mRNA-based assay services for our customers, which can solve your particular project requirements.
Nuclease protection assay is a powerful technique that can be used to identify, detect, and quantify individual RNA molecules in complex mixtures of total cellular RNA. The hybridization of probes to an RNA sample is the basic technology of nuclease protection assay in solution. Creative Biolabs provides both RNase protection assays and S1 nuclease protection assays, to identify one or more RNA molecules of known sequence even at the low total concentration for our customers.
PrimeFlow RNA Assay incorporates a proprietary oligonucleotide probe set design and branched DNA (bDNA) signal amplification technology to analyze RNA transcripts by flow cytometry. bDNA technology provides a unique approach to RNA detection and signals amplification by amplifying the reporter signal rather than the target sequence (e.g., PCR) for consistent results. Creative Biolabs is pleased to offer the PrimeFlow RNA assay to enable high-throughput detection of RNA and protein expression, which can be used to mechanistically and phenotypically characterize coexpression of RNAs with functional proteins at the single-cell level.
combination of high detection sensitivity, broad linear response range, and large fluorescence enhancement is available for sensitive fluorescence-based detection of RNA in the solution. Creative Biolabs can help our customers width reliable quantitation and detection of RNA by fluorescence assay using a standard fluorometer or fluorescence microplate reader to satisfy your project objective. With our standard assay conditions, the fluorescence assay offers accuracy and precision of measurement with a linear dynamic range.
RNA methylation is the most common and abundant modification in RNA molecules present in eukaryotes. RNA methylation assay is of great importance to understand RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. We provide a series of RNA methylation assays for our customers, including absorbance-based quantitation of N6-methyladenosine in RNA, fluorescence-based quantitation of 5-mC RNA methylation and chemical-based methylation assay.
A: They are used to detect, quantify, and analyze RNA molecules to understand gene expression, RNA stability, and protein interactions.
A: Techniques include Nuclease Protection Assay, PrimeFlow RNA Assay, and RNA Fluorescence Assay.
A: It uses oligonucleotide probes and bDNA technology for high-throughput RNA and protein expression analysis via flow cytometry.
A: It identifies, detects, and quantifies RNA molecules in complex RNA mixtures, even at low concentrations.
A: It evaluates gene expression by linking regulatory sequences to reporter genes like luciferase or GFP.
A: It measures the rate of mRNA degradation to study mRNA stability and gene expression regulation.
A: Stringent quality control measures ensure high accuracy, reliability, and reproducibility of results.
This article presents a protocol for identifying and characterizing HIV viral reservoirs. This protocol combines RNA fluorescent in situ hybridization (RNA FISH) with flow cytometry to detect HIV gag-pol mRNA and Gag protein simultaneously. It enhances sensitivity, detecting as few as 0.5–1 infected cell per million CD4 T cells. The method allows for detailed phenotyping of translation-competent viral reservoirs, including reactivated latent reservoirs in individuals undergoing antiretroviral therapy. This approach aids in understanding the persistence of HIV and assessing the effectiveness of potential cure strategies.
Fig. 1 Results of median fluorescent intensities (MFI) and frequencies of mRNA+ cells.1
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