Nucleic acid delivery's molecular pharmacology depends on nanocarrier surface properties. Zeta Potential defines colloidal stability and membrane interaction. Creative Biolabs' service offers precise measurement/kinetic analysis to optimize these parameters, backed by LNP-protein interaction literature.
Creative Biolabs provides a Custom Surface Charge Characterization Service. It optimizes stability/uptake via advanced Zeta Potential and binding analysis, delivers high-resolution data for formulation integrity and in vivo prediction, and defines nanocarrier physicochemical traits to meet stability/biological interaction criteria.
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| Zeta Potential (ZP) | A key indicator for measuring the electrokinetic potential on the particle surface. It directly reflects the strength of electrostatic repulsion between particles and serves as a core parameter for judging LNP colloidal stability. |
| Surface Charge Density | The amount of charge carried per unit area on the LNP surface. It quantifies the "density" of surface charge, and more intuitively reflects charge-carrying capacity compared to zeta potential. |
| Isoelectric Point (pI) | The pH value of the solution at which the net charge on the LNP surface is zero. It determines the charge state of LNPs in environments with different pH values, and is crucial for formulation pH optimization and in vivo targeting design. |
| Surface Charge Uniformity | Evaluates the degree of difference in surface charge among particles in an LNP population. Uneven charge distribution increases the risk of particle aggregation and affects formulation stability. |
Our workflow is designed for transparency and efficiency, ensuring every client understands the rigorous process involved in characterizing their unique formulation.
Check quality (e.g., DLS size) to qualify the sample, define parameters.
Use LDA to measure particle movement; get raw data.
Apply the equation to get ZP value (mV); core electrostatic measure.
Map stability via ZP across pH/ionic strengths; find optimal window.
Use SPR to get KD, ka, and kd for biodistribution prediction.
As leading experts in nanomedicine surface science, Creative Biolabs offers a dedicated, consultative service model built around your specific therapeutic goals. We don't just provide data; we provide customized, regulatory-ready surface intelligence to propel your drug development forward.
Customized Environment Mimicry
Analytical protocols are meticulously tailored (e.g., buffer pH, osmolarity, specific serum protein spiking) to precisely reflect the target delivery tissue or final drug product storage conditions.
Multimodal Surface Intelligence
Comprehensive, one-stop characterization combining static charge (Zeta Potential) and dynamic interaction (SPR kinetics) for the most complete predictive surface profile.
Regulatory-Centric Quality System
Our entire workflow adheres to Quality-by-Design (QbD) principles, ensuring all data generated is high-integrity, traceable, and suitable for direct inclusion in regulatory filings.
Adaptability Across Nanocarriers
Proven capability to accurately characterize diverse cationic delivery systems, including lipid nanoparticles, polyplexes, and liposomes, across various nucleic acid payloads (mRNA, siRNA, oligonucleotide).
Predictive Species Cross-Reactivity
Custom binding screens against ApoE homologs from human, rodent, and primate sources to de-risk preclinical translation by validating your chosen animal model in vitro.
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To evaluate the average hydrodynamic diameters of two LNP preparations before and after encapsulating RNA cargo, and the Zeta potential measurements before and after encapsulating RNA cargo at three different pH values.
Fig.1 Formulation and characteristic detection of LNP.1
Encapsulation efficiency only tells you how much payload is inside; Zeta Potential measures the outer surface charge, which governs the particle's stability in solution and its ability to enter the cell. A high-encapsulation LNP with a poor Zeta Potential will aggregate quickly or be rejected by the cell membrane, making the payload effectively useless in vivo. We help you optimize both.
Yes. While final organ tropism is complex, the Biomolecular Corona is the key determinant. We use SPR binding assays to measure the affinity of your LNP for crucial serum proteins like ApoE. High ApoE affinity is a strong predictor of liver (hepatic) targeting via the LDLR pathway, giving you a powerful, early-stage predictive tool.
The most critical precaution is the dependency on the medium. The Zeta Potential value will change drastically based on the pH and salt concentration of the buffer. Our reports are specific, and we recommend always using a buffer that closely simulates your target physiological or storage condition for meaningful data interpretation.
DLS (Dynamic Light Scattering) measures particle size and distribution (polydispersity), which is an indirect measure of stability. SPR is fundamentally different: it is a direct, kinetic measurement of biomolecular interaction. It tells you how strongly your LNP binds to a target protein, which DLS cannot do. Using both provides a complete picture of your formulation's quality.
Absolutely. Highly positive LNPs can sometimes lead to increased systemic toxicity or non-specific binding. Our characterization workflow, particularly the pH titration aspect, helps identify the optimal formulation ratio that yields a near-neutral charge in circulation but still possesses the optimal pKa for triggered endosomal release, helping you balance efficacy and tolerability.
Creative Biolabs' analytical intelligence is required to develop stable, targeted, and highly efficacious mRNA nanotherapeutics. By combining gold-standard Zeta Potential measurement for stability assurance with advanced SPR binding kinetics for biodistribution prediction, we furnish your project with the quantitative data required for confident progression toward the clinic.
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