Tribehenin, C69H134O6, is consists of glycerin and behenic acid. It is a naturally occurring long-chain fatty acid, which is found in vegetable and animal fats and oils and used as a conditioner in many kinds of skin care products.
Solid lipid nanoparticles (SLNs) composed of tristearin (TS) or tribehenin (TB), and monoolein aqueous dispersions (MADs) composed of glyceryl-monoolein have been studied as potential nucleic acid nanocarriers. Cationic surfactants such as diisobutylphenoxyethyl-dimethylbenzyl ammonium chloride (DEBDA) or PEG-15 Cocopolyamine (PCPA) were added to the lipid composition to obtain the cationic properties of the nanocarriers. These nanosystems can bind nucleic acid molecules to form stable complexes with DNA, and the research showed that the produced nanocarriers can deliver plasmids into cells. The development of safe and efficient non-viral systems for gene delivery is a major challenge in gene therapy. SLN and MAD are obtained using physiologically well-tolerated ingredients that are already approved for pharmaceutical use in humans.
Fig.1 Cryo-transmission electron microscopy images (Cryo-TEM) of cationic SLN dispersions. (A) TS-DEBDA 1% SLN. (B) TB-DEBDA 1% SLN. The inset shows one particle side viewed. (C) TS-PCPA 1.5% SLN. (D) TB-PCPA 1.5% SLN. Bar represents 0.2 mm. (Cortesi, 2014)
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