RNA binding protein (RBPs) plays an important role in post-transcriptional regulation, such as the regulation of mRNA splicing, translation and degradation in different biological environments. The recognition and characterization of RNA substrate is the key step to decipher the function and molecular mechanism of the target RBPs.
The high-throughput sequencing of RNA fragments isolated by crosslinking immunoprecipitation (CLIP-seq) is one of the standard techniques for the identification of wide binding sites in the transcriptome of target RNA binding proteins. Using this technique, it is possible to label the natural binding site of RBPs, and may allow the identification of the entire target library of RBPs, thereby contributing to a better understanding of post-transcriptional regulatory networks and mechanisms.
CLIP-seq, also known as HITS-CLIP, combines UV cross-linking and immunoprecipitation with high-throughput sequencing to identify the binding site of RBPs. CLIP-seq depends on the crosslinking induced mutation site (CIMS) and the local protein-RNA binding site. Because CIMS is reproducible, a higher sequencing depth can distinguish CIMS from technical errors.
The workflow of CLIP-seq is as follows:
Creative Biolabs has more than 10 years of experience in the field of RNA-protein interaction research, our existing technology provides a flexible platform for high-throughput biochemistry, which can be easily extended to any nucleic acid template to study different types of biochemical interactions. At Creative Biolabs, we accept a wide range of biological and clinical samples, including cells and tissues. You only need to provide samples, and we will design and complete a complete CLIP-seq analysis according to your project needs. The complete report will provide you with raw and analytical data, charts, detailed protocols, and data analysis reports. For specific analysis, please do not hesitate to contact us for more details.