Gene therapy is a therapeutic approach designed to transfer exogenous genetic material, such as mRNA, into cells in order to correct a genetic defect or induce the expression of a specific desired protein. It's very powerful because this technology can be used to correct inherited diseases or treat diseases with a relative understanding of pathophysiology. However, the most important problem in gene therapy is to develop an efficient, safe and convenient gene vector. The appearance of lipopolyplex provides a new way to solve this problem.
Lipopolyplex is a ternary complex of cationic liposome, polycation and nucleic acid. It is the second generation of non-viral gene vector developed after the first generation of cationic liposome complex. Specifically, mRNA molecules are encapsulated in a polymer core loaded into a phospholipid bilayer shell structure. The obtained structure can not only protect the mRNA molecules in the multi-node core structure from the attack of RNA enzyme, but also be internalized effectively by dendritic cells (DCs). In DCs, particles are transported through the vector system, and the mRNA molecules are released into the cytoplasm to generate antigens.
Fig. 1 Schematic view of the Lipopolyplex mRNA.1
Lipopolyplex combines the advantages of polyplex and lipoplex. Due to their synergistic effect, lipopolyplex shows superior colloidal stability, lower cytotoxicity and high gene transfection efficiency. There are many strategies based on lipopolyplex to overcome the complex biological vectors in gene delivery, including:
Fig. 2 Intracellular barriers for lipopolyplexes-mediated gene delivery.2
As an innovative, promising and DNA-based alternative therapy strategy, mRNA-based therapy has attracted widespread attention. At present, Scientists of Creative Biolabs focus on the delivery system of nucleic acids, and have developed the most advanced, non-viral, self-assembled complex vector for mRNA. Lipopolyplex is one of them. Our lipopolyplex vectors provide efficient and repeatable strategies for mRNA delivery to a variety of cells. This delivery mechanism simulates mRNA delivery in several key ways, including active entry (endocytosis), homeostasis, and release.
Relying on the experience in gene therapy research, Creative Biolabs is committed to developing and commercializing vector systems for mRNA delivery. Our long-term goal is to become a leader in the development and commercialization of technologies that will accelerate and enhance discoveries of gene delivery systems for gene therapy research and applications. We are committed to providing quality products and services to customers from all over the world, and maintaining our commitment to quality, price, service and customer relationships.
Inquire About Our ServicesA: Lipopolyplexes are complex structures composed of lipids, polymers, and nucleic acids (such as DNA or RNA). They are used in drug delivery to enhance the stability, cellular uptake, and transfection efficiency of nucleic acids. The combined properties of lipids and polymers in lipopolyplexes provide improved protection and delivery of genetic material to target cells.
A: Creative Biolabs offers comprehensive lipopolyplex preparation services, including the formulation and optimization of lipopolyplexes, characterization of physical and chemical properties, assessment of encapsulation efficiency, stability testing, and evaluation of in vitro and in vivo delivery efficacy.
A: Creative Biolabs optimizes lipopolyplex formulations by adjusting the ratios of lipids, polymers, and nucleic acids to achieve the best balance of stability, delivery efficiency, and biocompatibility.
A: It offers superior colloidal stability, lower cytotoxicity, and high gene transfection efficiency.
A: They provide efficient and repeatable mRNA delivery strategies using advanced non-viral, self-assembled vectors.
A: Condensation of nucleic acids, long circulation, cell targeting, endosomal escape, release to cytoplasm, and entry into the cell nucleus.
This study designed a lipopolyplex mRNA vaccine that enhances anti-tumor immunity. The lipopolyplex consists of an mRNA core made from poly-(β-amino ester) polymer and encapsulated within a lipid shell of EDOPC/DOPE/DSPE-PEG. This structure facilitates efficient entry into dendritic cells via macropinocytosis and protects mRNA from enzymatic degradation. The vaccine showed significant adjuvant activity by stimulating the production of interferon-γ and interleukin-12 via Toll-like receptor 7/8 signaling, enhancing antigen presentation. In a mouse model with lung metastatic B16-OVA tumors, the lipopolyplex demonstrated potent anti-tumor effects, suggesting its potential as an effective mRNA vaccine delivery system.
Fig.3 Anti-tumor activity from LPP/mRNA in vitro and in vivo.1
| Cat. No | Product Name | Promoter |
|---|---|---|
| CAT#: GTVCR-WQ001MR | IVTScrip™ pT7-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ002MR | IVTScrip™ pT7-VEE-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ003MR | IVTScrip™ pT7-VEE-mRNA-FLuc Vector | T7 |
| CAT#: GTVCR-WQ87MR | IVTScrip™ pT7-VEE-mRNA-Anti-SELP, 42-89-glycoprotein Vector | T7 |
| Cat. No | Product Name | Type |
|---|---|---|
| CAT#: GTTS-WQ001MR) | IVTScrip™ mRNA-EGFP (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| CAT#: GTTS-WK18036MR | IVTScrip™ mRNA-Human AIMP2, (Cap 1, Pseudo-UTP, 120 nt-poly(A)) | Enzyme mRNA |
| (CAT#: GTTS-WQ004MR) | IVTScrip™ mRNA-Fluc (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| (CAT#: GTTS-WQ009MR) | IVTScrip™ mRNA-β gal (Cap 1, 30 nt-poly(A)) | Reporter Gene |
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