Creative Biolabs is a leading service provider that focuses on mRNA-based assays. Along with over a decade of extensive experience in providing excellent services for RNA methylation assay, we have won a great reputation among our worldwide customers for accomplishing numerous challenging projects.
RNA methylation is a reversible post-translational modification to RNA that plays a significant role as an epigenetic mechanism and epigenetically impacts numerous biological processes. It occurs in different RNAs, including tRNA, rRNA, mRNA, snRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases. N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes and accounts for more than 80% of all RNA base methylations and exists in various species. 5-methylcytosine (5-mC) is an epigenetic mark that also commonly occurs in various RNA molecules. Recent studies have shown that m6A and 5-mC RNA methylation is important in the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to the etiology of human diseases. Different approaches have been developed in order to map and quantify RNA methylation marks.
Fig. 1 RNA methylation.1
The relative abundance of m6A/5-mC in mRNA transcripts affects RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. The dynamic and reversible chemical m6A/5-mC modification in RNA may also serve as a novel epigenetic marker of profound biological significance. Moreover, it has been shown that this modification plays an important role in the development of several disorders, such as neuronal disorders, immune-mediated diseases, obesity, and cancer. Therefore, more useful information for a better understanding of m6A RNA methylation levels and distribution on RNA transcripts could benefit diagnostics and therapeutics of disease. The RNA methylation quantification assay is a complete set tool to colorimetrically or fluorometrically quantify m6A/5-mC in RNA. It is suitable for direct detection of m6A/5-mC RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses.
In this assay, total RNA is bound to strip wells, and m6A/5-mC is detected using a specific capture m6A/5-mC antibody and detection antibody. The detected signal is enhanced and then quantified colorimetrically or fluorometrically by reading the absorbance or fluorometric. The amount of m6A/5-mC is proportional to the intensity measured. A standard curve can be performed, or a single quantity of m6A/5-mC can be used as a positive control. Because m6A/5-mC content can vary from tissue to tissue, and normal and diseased states, or vary under-treated and untreated conditions, replication samples can be used to ensure that the signal generated is validated.
As a top-ranking provider in the RNA market, Creative Biolabs offers various assays to deal with the urgent demands for more RNA detection. Our high-quality RNA methylation assay services will greatly contribute to the success of your projects. If you want to know more information about our services, please do not hesitate to contact us.
Reference
