mRNA cap is a unique mark that involves specific mRNA processing and translation initiation. Many mRNA studies require reliable preparation and modification of 5'-capped RNAs. As a customer-oriented CRO company, Creative Biolabs provides a comprehensive range of customized, high-quality mRNA services in vaccine and therapeutics development to support the pharmaceutical industry and related biomedical sciences research communities worldwide.
The classical cap structure is a 7-methylguanosine moiety connected to the mRNA via a triphosphate linkage at 5'-end (m7GpppG or m7G). It can be recognized by several specialized proteins involved in mRNA transport, translation, translational repression and degradation. 6-thioguanosine (s6G) is a photosensitive molecule that can photo-crosslink to both proteins and nucleic acids to study cap-protein and cap-nucleic acid interactions at the molecular level.
s6G is suitable for the preparation of capped RNAs by in vitro transcription (IVT) because this modification into mRNA cap confers resistance to Dcp1/2 (mRNA decapping) and stabilizes interaction with eIF4E, resulting in increased translational efficiency and half-life of mRNA. Moreover, s6G modification is a useful tool for structural and functional studies of RNAs, including the activity of ribonuclease P, interaction of cap analogs, triplexes formation and RNA sequencing and population dynamics.
Fig.1 6-Thioguanosine capping.
Creative Biolabs is committed to meeting your specific requirements for synthetic mRNA. We have developed one all-around platform for mRNA in vitro transcription, mRNA modification, mRNA delivery, mRNA stability test, mRNA structure prediction, mRNA-based cell reprogramming, etc. Our high-quality services address the unmet needs in synthetic mRNA for rapidly growing research and clinical uses. For mRNA modification, we not only provide 5' capping and 3' poly (A) tailing service, but also provide 5' or 3' UTR (Kozak sequence) for improved translation efficiency. Especially, we provide two capping options for the covalent linkage of s6G to RNA for your choice:
Fig.2 Structure of cap analogs containing 6-thioguanosine. (Nowakowska, 2014)
Cap analogs containing s6G moieties serve as attractive molecular tools for photo-crosslinking experiments on RNA interactions study. Creative Biolabs offers a comprehensive range of mRNA services characterized by quality, predictability, delivery, and customer service. If you would like to discuss available development options, please feel free to contact us.