The classical cap structure is a 7-methylguanosine moiety connected to the mRNA via a triphosphate linkage at 5'-end (m7GpppG or m7G). It can be recognized by several specialized proteins involved in mRNA transport, translation, translational repression and degradation. 6-thioguanosine (s6G) is a photosensitive molecule that can photo-crosslink to both proteins and nucleic acids to study cap-protein and cap-nucleic acid interactions at the molecular level.
s6G is suitable for the preparation of capped RNAs by in vitro transcription (IVT) because this modification into mRNA cap confers resistance to Dcp1/2 (mRNA decapping) and stabilizes interaction with eIF4E, resulting in increased translational efficiency and half-life of mRNA. Moreover, s6G modification is a useful tool for structural and functional studies of RNAs, including the activity of ribonuclease P, interaction of cap analogs, triplexes formation, RNA sequencing, and population dynamics.
Fig.1 6-Thioguanosine capping.
Creative Biolabs is committed to meeting your specific requirements for synthetic mRNA. We have developed one all-around platform for mRNA in vitro transcription, mRNA modification, mRNA delivery, mRNA stability test, mRNA structure prediction, mRNA-based cell reprogramming, etc. Our high-quality services address the unmet needs in synthetic mRNA for rapidly growing research and clinical uses. For mRNA modification, we not only provide 5' capping and 3' poly (A) tailing service, but also provide 5' or 3' UTR (Kozak sequence) for improved translation efficiency. Especially, we provide two capping options for the covalent linkage of s6G to RNA for your choice:
Fig.2 Structure of cap analogs containing 6-thioguanosine.1
Inquire About Our ServicesA: It is a modified mRNA cap incorporating 6-thioguanosine (s6G), used to enhance mRNA stability and study mRNA interactions.
A: s6G increases mRNA stability, resists decapping enzymes, and facilitates photo-crosslinking to study RNA-protein and RNA-nucleic acid interactions.
A: Applications include RNA interaction studies, structural and functional RNA research, and improved mRNA stability for therapeutics.
A: It can be incorporated either co-transcriptionally or post-transcriptionally using enzymatic labeling techniques.
A: High-performance liquid chromatography (HPLC) and mass spectrometry are used for verification.
A: They provide comprehensive consultation, design, and optimization services tailored to client needs.
The article investigates the effects of incorporating 6-thioguanosine (s6G) into RNA duplexes. It explores how s6G influences the thermodynamic stability and structural properties of RNA. The study reveals that s6G generally decreases the stability of RNA duplexes, though the extent of destabilization depends on its position within the sequence. When s6G is located at terminal positions, it has a weaker destabilizing effect compared to internal positions. Despite these changes in stability, structural analysis using NMR and circular dichroism indicates that the overall RNA duplex structure remains largely unaffected. These findings are significant for understanding RNA behavior and designing RNA-based therapeutics and research tools.
Fig.3 NMR analysis of s6G-containing RNA duplexes.2
| Cat. No | Product Name | Promoter |
|---|---|---|
| CAT#: GTVCR-WQ001MR | IVTScrip™ pT7-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ002MR | IVTScrip™ pT7-VEE-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ003MR | IVTScrip™ pT7-VEE-mRNA-FLuc Vector | T7 |
| CAT#: GTVCR-WQ87MR | IVTScrip™ pT7-VEE-mRNA-Anti-SELP, 42-89-glycoprotein Vector | T7 |
| Cat. No | Product Name | Type |
|---|---|---|
| CAT#: GTTS-WQ001MR) | IVTScrip™ mRNA-EGFP (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| CAT#: GTTS-WK18036MR | IVTScrip™ mRNA-Human AIMP2, (Cap 1, Pseudo-UTP, 120 nt-poly(A)) | Enzyme mRNA |
| (CAT#: GTTS-WQ004MR) | IVTScrip™ mRNA-Fluc (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| (CAT#: GTTS-WQ009MR) | IVTScrip™ mRNA-β gal (Cap 1, 30 nt-poly(A)) | Reporter Gene |
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