Creative Biolabs specializes in the Custom Chemical Synthesis of mRNA, utilizing advanced technology and a team of seasoned experts to craft bespoke, short-length mRNAs tailored to each client's unique specifications. As a global leader in mRNA technology, we commit to accelerating client projects by providing top-tier chemical synthesis services that ensure precise and efficient results. Our approach combines extensive experience in mRNA handling with cutting-edge instruments to deliver custom solutions that fulfill specific research and development needs. Whether for therapeutic applications or research purposes, our services are designed to support projects of any scale effectively. For detailed inquiries and to receive a tailored quotation, please reach out to our customer support team via email. We are ready to assist you promptly and help advance your projects with our reliable and professional services.
Modified mRNAs can readily be obtained from any DNA template using RNA polymerases in a short time. However, mRNA prepared by enzymatic synthesis may have transcription errors that allow it to be produced only on a laboratory scale, and the product may not be pure enough for its intended use. An efficient scalable non-enzymatic (chemical) method for mRNA production could solve these problems. Chemical synthesis of single-stranded mRNA molecules is a widely used laboratory procedure that is an alternative strategy of mRNA synthesis for RNA research as well as RNA biopharmaceuticals. This technique is versatile and allows the researcher to tailor the synthesis of short fragments of mRNA and introduce modifications to mRNA.
Fig.1 Structural mRNA feature.
Chemical-synthesized RNA can be used for the development of mRNA vaccines, genome editing tools, and mRNA amplification-based diagnostics. Creative Biolabs can assemble mRNA strands by solid-phase synthesis and generate a large number of modified RNAs that can be further improved in purity by conventional methods. According to customer needs, we can provide custom mRNA chemical synthesis services, a wide range of post-transcriptional modifications, various purification options, and suites of analytical services. Additionally, our comprehensive quality control system ensures a robust manufacturing process to provide our customers with excellent and consistent lot-to-lot quality.
Inquire About Our ServicesA: When your project requires highly customized sequences, specific chemical modifications, or mRNA of extremely high purity and consistency, chemical synthesis of mRNA is a superior choice. Although enzymatic synthesis is cost-effective and suitable for large-scale production, it may not meet the specific requirements mentioned above in certain specialized applications.
A: Creative Biolabs can synthesize mRNA of various lengths, tailored to client needs, typically up to several kilobases.
A: We can provide modifications such as capping, tailing, and incorporation of modified nucleotides according to the unique needs of our clients to enhance the stability and translation efficiency of mRNA.
A: Purification methods include high-performance liquid chromatography (HPLC) and gel electrophoresis to ensure high purity and quality.
A: We provide consultation on mRNA design, synthesis optimization, and troubleshooting to meet specific research and therapeutic needs.
This article details the development and synthesis of artificial mRNA using a novel chemical approach. This method uses 2-cyanoethoxymethyl as a protecting group and highly reactive phosphotriester pyrophosphorylating reagents to produce a 130-nucleotide mRNA encoding glucagon-like peptide-1 (GLP-1). The synthetic mRNA, capped and polyadenylated enzymatically, demonstrated biological activity by supporting protein synthesis in cell-free systems and in living cells. This research presents a scalable method for producing high-purity mRNA, offering potential for therapeutic applications and mRNA-based vaccines.
Fig.2 Polyacrylamide gel electrophoresis of the poly(A)-tailing reaction.1
| Cat. No | Product Name | Promoter |
|---|---|---|
| CAT#: GTVCR-WQ001MR | IVTScrip™ pT7-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ002MR | IVTScrip™ pT7-VEE-mRNA-EGFP Vector | T7 |
| CAT#: GTVCR-WQ003MR | IVTScrip™ pT7-VEE-mRNA-FLuc Vector | T7 |
| CAT#: GTVCR-WQ87MR | IVTScrip™ pT7-VEE-mRNA-Anti-SELP, 42-89-glycoprotein Vector | T7 |
| Cat. No | Product Name | Type |
|---|---|---|
| CAT#: GTTS-WQ001MR) | IVTScrip™ mRNA-EGFP (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| CAT#: GTTS-WK18036MR | IVTScrip™ mRNA-Human AIMP2, (Cap 1, Pseudo-UTP, 120 nt-poly(A)) | Enzyme mRNA |
| (CAT#: GTTS-WQ004MR) | IVTScrip™ mRNA-Fluc (Cap 1, 30 nt-poly(A)) | Reporter Gene |
| (CAT#: GTTS-WQ009MR) | IVTScrip™ mRNA-β gal (Cap 1, 30 nt-poly(A)) | Reporter Gene |
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