Creative Biolabs is one of the well-recognized experts who are professionals in the field of mRNA delivery. Now, we are proud to offer a series of reagents for mRNA delivery, such as waxes (cetyl palmitate).
Solid Lipid Nanoparticles (SLN) in Drug and Gene Delivery Systems
Various non-viral delivery systems have been demonstrated to efficiently deliver small-interfering RNAs (siRNAs) and DNA, including cationic polymers, liposomes, cationic lipids, cell-penetrating peptides, and so on. SLNs as gene delivery systems have attracted increasing attention in recent years. SLNs are the next generation of lipid nanoparticles after liposomes. SLNs have been considered an efficient and non-toxic alternative lipophilic colloidal carrier for the delivery of various drugs and other biological macromolecules like DNA and peptides. SLNs are biodegradable and stable for a long time and are easy to scale up compared to other colloidal systems.
Fig.1 Fields of application of SLNs. (Carbone, 2014)
Solid lipid, emulsifier and water/solvent have been used in the preparation of SLNs. The lipids used may be triglycerides, partial glycerides, fatty acids, steroids, and waxes (e.g., cetyl palmitate). Cetyl palmitate, which is biocompatible and non-toxic to human cells, can be chosen as the biocompatible core lipid. The composition of the SLN dispersions is selected based on encouraging previous experience with cetyl palmitate and phospholipids-surfactants regarding the formation of particles with good physical stability. Also, the long hydrophobic chain fatty acids of cetyl palmitate can retain lipophilic drugs resulting in sustained drug release.
Researchers have developed the SLN-siRNA for targeted therapy in which SLNs are prepared with cetyl palmitate using a modified method of the melt-emulsification technique. First, an oil in water (o/w) microemulsion system is formed using cetyl palmitate as internal oil phase, Cremephor RH40, Peceol as surfactants, and propylene glycol as cosurfactant. Following the construction of the phase diagram, a microemulsion consisting of oil, surfactant/cosurfactant, and water is found to yield a transparent formulation and is selected for further studies.
Creative Biolabs focuses on the SLN systems fabricated from biocompatible ingredients-cetyl palmitate and co-loaded with the therapeutic agents. With over a decade of extensive experience, we offer a series of products for your specific demands. If you are interested in our reagents, please do not hesitate to contact us for more detailed information.
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