Creative Biolabs has been a long-term expert in the field of mRNA therapeutics. To achieve efficient and safe delivery of mRNA, now we are proud to offer a series of reagents, especially Dimethyl Dioctadecyl Ammonium Bromide (DDAB).
Dimethyl Dioctadecyl Ammonium Bromide (DDAB), also known as Dioctadecyl Dimethyl Ammonium Bromide (DODAB), is a double-chained quaternary ammonium surfactant that can form unilamellar vesicles (ULVs) in water.
In recent years, studies have shown that permanently cationic, zwitterionic/anionic, or ionizable cationic lipids selectively induce mRNA delivery to the lung, spleen, or liver, respectively.
Dimethyl Dioctadecyl Ammonium Bromide (DDAB) is a common quaternary ammonium compound as well as a permanently cationic lipid. It can form a complex with mRNA and can also stimulate the innate immune response, thereby acting as an immune adjuvant for mRNA vaccines. Compared with DOTAP or DOPE, DDAB more stably binds with tRNA. In this case, DDAB can more stably promote the delivery of siRNA to tumor cells. In addition, DDAB and DOPE constitute the commercial product TransfectAce.
Using a film-ultrasonic method, solid lipid nanoparticles (SLNs) containing DDAB can be constructed and have been applied for miR-34a mimics delivery into the cancer stem cells to that increase the survival rate of CSC-bearing mice. As a cationic surfactant, DDAB presents genotoxic effects in mammalian cells at environmentally relevant concentrations, more research is still required to reduce the toxicity and improve the stability.
Fig 1. Physiological barriers for lipid nanoparticle-mRNA (LNP-mRNA) formulations post systemic and local delivery. (Hou, 2021)
As a pioneer and the undisputed global leader in mRNA therapeutics, we are pleased to use our extensive experience and advanced platform to offer the best service and the most qualified products to satisfy each demand from our customers. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.
Reference
