Differential scanning calorimetry (DSC) techniques could be useful to provide new data about the structural thermodynamics of reaction intermediates. It is a powerful technique for obtaining the thermoanalytical parameters of biomolecules and nanomaterials. Creative Biolabs acts as an innovator and problem solver in the mRNA delivery industry, we can offer custom solutions based on DSC technology.
DSC is a thermodynamical tool for direct assessment of the heat energy uptake, which occurs in a sample within a regulated increase or decrease in temperature. As a powerful analytical tool, DSC is capable of elucidating the factors that contribute to the folding and stability of biomolecules. Among various analytical techniques, DSC has been used for quantifying an amorphous or crystalline phase in nano solids. Several methodologies based on the calorimeter type (conventional DSC or MTDSC), measured parameters, and experimental conditions are used for monitoring the behavior of pharmaceutical nano solids.
Based on the experiences with hard fats nanoparticles, a new type of SLN has been developed by incorporating triglyceride-containing oils in the solid core of the said particle. Nanoparticles were characterized by their melting and recrystallization behavior as recorded by DSC. DSC gives an insight into the melting and recrystallization behavior of crystalline material like lipid nanoparticles. Fig.1 gives an overview of the melting and crystallization process of a 15% suspension of nanoparticles in surfactant solution. The nanoparticles were prepared with different ratios of liquid to solid lipids. The depression of the onset and the temperature of the peak maximum depended on the oil concentration in an approximately linear fashion for the given concentration range. The DSC experiments revealed polymorphism for Compritol nanoparticles as well. In addition to the main peak, a peaked shoulder at higher temperatures was observed. In addition to the characterization of the solid material of the carrier, DSC can be employed to study the liquid phase within the nanoparticles. DSC measurements showed that both components exhibit good miscibility in the nanoparticulate state and formed homogenous particles.
Fig.1 DSC scans of SLN dispersions containing (from top) 0, 8, 16, 28, 33, and 38% Miglyol (heating from 25 to 85oC and subsequent cooling from 85 to 5 oC at a rate of 5 K/min). Dark triangles point at minor amounts of a second (and third) polymorph of Compritol. (Jenning, 2000)
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