Laser Diffraction

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Creative Biolabs specializes in mRNA delivery research and has developed a wide range of methods for the characterization of lipid nanoparticles to provide customized services during mRNA delivery project implementation. Laser diffraction (LD) analysis provides a rapid, cost-effective, and precise method of undertaking particle size analysis. Here are some application cases of this technology.

Preparation and Characterization of Solid Lipid Nanoparticles (SLN) Loaded Traditional Chinese Medicine

  • Average Diameter of SLN

Ultrasonication was employed to prepare SLN. The model of traditional Chinese medicine, tetrandrine (TET), was incorporated into SLN. An adequate characterization of the solid lipid nanodispersion is a necessity for the control of the quality of the product. LD is one of the most powerful techniques for routine measurements of particle size. The average diameter of TET-SLN measured by LD was 157.3±8.2 nm (n=9). The drug loading SLN showed a considerably small particle size. Stability evaluation showed relatively long-term stability with only slight particle growth (P > 0.05) after storage at room temperature for 4 weeks. Therefore, ultrasonication is demonstrated to be a simple, available, and effective method to prepare high-quality SLN loaded traditional Chinese medicine.

TEM photograph of TET-SLN (×40,000).Fig.1 TEM photograph of TET-SLN (×40,000). (Li, 2006)

Determination of Particle Shape Information From LD

The particle image contour lines are usually either compressed by using shape factors such as the fractal dimension, the aspect ratio, or the elongation, or they are transformed by Fourier analysis into a set of Fourier coefficients to reflect the particle. There are two direct ways of obtaining particle shape information from LD-type measurements. The first one uses the changes in the angular scattering pattern for agglomerating, monodisperse particles to derive the fractal dimension. This method seems to be limited to narrow size distributions of primary particles. The second one can be applied more generally and makes use of the intensity differences of the scattered light at azimuthal angles for non-spherical particles. For this purpose, a wedge type of detector can be used.

Wedge-type photo detector.Fig.2 Wedge-type photo detector. (Ma, 2000)

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References

  1. Li, Y.; et al. Preparation and characterization of solid lipid nanoparticles loaded traditional Chinese medicine. International journal of biological macromolecules. 2006, 38(3-5): 296-299.
  2. Ma, Z.; et al. New developments in particle characterization by laser diffraction: size and shape. Powder Technology. 2000, 111(1-2): 66-78.
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