Nuclear Magnetic Resonance

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Creative Biolabs is a global leader in the rapidly emerging market for mRNA. With our powerful nuclear magnetic resonance (NMR) technology, we can characterize lipid nanoparticles (LNPs) to study the interactions of the nanomaterials with the ligands and other counterparts for your specific needs.

LNPs are colloidal lipidic systems that have been proposed for drug delivery system candidates with promising applications for siRNA and mRNA delivery. LNPs include solid lipid nanoparticles (SLNs) and oil-loaded SLN (also described as nanostructured lipid carriers-NLCs). SLNs are aqueous colloidal dispersions with a size in the range of 50-1000 nm; the matrix comprises biodegradable and biocompatible solid lipids. LNPs have many advantages, including providing controlled drug release and drug targeting, protecting incorporated drugs against chemical degradation, biosafety of the carrier, and the feasibility of large-scale production.

What is NMR?

NMR spectroscopy is a powerful analytical characterization technique, which has been exploited to determine constituents involved in nanoencapsulation systems. NMR provides versatile chemical, dynamic and spatial information of molecules in solution non-invasively. NMR spectroscopy with vivid experiments in the form of liquid state and solid-state, one-dimensional (1D), two-dimensional (2D), and multidimensional (nD), can provide a deep insight into molecular weight, purity, composition, structure, dynamics, diffusion properties, the stoichiometry of nano-materials.

The principle of the NMR experiment.Fig.1 The principle of the NMR experiment. (Agarwal, 2018)

NMR for LNPs Characterization

The NMR techniques provide many prospects for characterization of the nanomaterials and their surface-bound ligands for the comprehensive set of parameters that include shape, size, morphology, spatial arrangement, affinity, and stoichiometry. Using NMR, we could determine both the size and the qualitative nature of LNPs and provide information on the physicochemical status of components within the LNPs.

Our seasoned scientific team can apply a combination of 2D NMR techniques to understand the structure, interactions, and dynamics/motilities of nanoparticles and nanomaterials. NMR studies can quantify the extent of particle fluidity, which provides further evidence on the localization of molecules within NLCs. This method allows correlating the molecular dynamics of lipids with lipid fluidity changes because NMR signals reflect the chemical environments of atoms. Moreover, solid-state NMR has been used to study the core-shell structure of LNPs composed of a variety of inorganic and carbon-based materials. It has also been used extensively to study the structure and dynamics of ligands or catalytic substrates on the surface of LNPs.

If you are interested in our technologies and mRNA solutions, please do not hesitate to contact us for more detailed information.

Reference

  1. Agarwal, N.; et al. Characterization of nanomaterials using nuclear magnetic resonance spectroscopy. In Characterization of nanomaterials. Woodhead Publishing. 2018, pp. 61-102.
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